Calibration is the process of aligning your specific printer to the specific lens being printed. If the image printing and mounting is outsourced, there is no need to calibrate to simply create the interlaced lenticular image.
To ensure the cleanest flip and best alignment you should calibrate often. Tempurature and humidity changes will affect the size of your lens and paper stability. The penalty for not aligning is uneven flips and poor image quality.
The calibration is an iterative cycle. That is, you must repeat it until you get within your tolerance. Depending on your lens and printer this can take some time. With patience the system becomes easy, and sometimes addictive: honing in on the perfect LPI number.
Examine each output option carefully and set as needed.
This number is equal to the number of lenses in each inch according to your printer. To obtain this continue to cycle through this process until finding a number within your tolerance. Initially, set this to the Lens Per Inch specified your lens manufacturer.
The number of pitch test strips to generate per image. they will be stretched and evenly spaced along the length of the image.
Increment by The amount the pitch will vary by for each strip. The setting in the Lens LPI field will be the center strip, this should be set to your best guess.
This is the DPI you want to print the image at. Run tests to ensure your printer prints well at this resolution. You will be able to create a larger image with less dpi, but you may find it not so. Test the image. More information is under the Calibration section.
Set this to the desired output size.